rat brain mrna (TaKaRa)
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TaKaRa
rat brain mrna
Rat Brain Mrna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+brain+poly+a+rna/Rat+Brain+Poly+A%2B+RNA/pmc03399833-153-7-15
Average 90 stars, based on 96 article reviews
Rat Brain Mrna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+brain+poly+a+rna/Rat+Brain+Poly+A%2B+RNA/pmc03399833-153-7-15
Average 90 stars, based on 96 article reviews
rat brain mrna - by Bioz Stars,
2026-10
90/100 stars
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Amplification:Article Title: Glutamate transporter associated proteins and methods of use thereof Article Snippet: Marathon cDNA amplification (CLONTECH) was used to perform both 5′- and 3′-RACE on cDNA synthesized from rat brain poly(A)+ RNA. .. Marathon cDNA amplification (CLONTECH) was used to perform both 5′- and 3′-RACE on cDNA synthesized from Article Title: RFRP-3 and DNA thereof Article Snippet: .. A cDNA encoding a bovine-type physiologically active peptide was obtained from Article Title: Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats. Article Snippet: .. The entire open reading frame of Kv1.2 and Kv1.5 was amplified from Synthesized:Article Title: Glutamate transporter associated proteins and methods of use thereof Article Snippet: Marathon cDNA amplification (CLONTECH) was used to perform both 5′- and 3′-RACE on cDNA synthesized from rat brain poly(A)+ RNA. .. Marathon cDNA amplification (CLONTECH) was used to perform both 5′- and 3′-RACE on cDNA synthesized from Reverse Transcription Polymerase Chain Reaction:Article Title: Development of a beta-lactamase reporter gene assay for metabotropic glutamate receptor 1 by using coexpression of glutamate transporter. Article Snippet: Metabotropic glutamate receptors (mglurs) belong to a family of G-protein-coupled receptors and are thought to be involved in the modulation of neuronal excitability and neurotransmitter release.. Eight subtypes of the receptor (mGluR1-mGluR8) have been identified to date and are classified into 3 groups based on sequence homology, pharmacological profile, and signal transduction pathway. mGluR1 and mGluR5 are classified into group I mGluRs and are coupled to phospholipase C and subsequent intracellular calcium mobilization via Gq-protein.. Earlier efforts to identify an mGluR1-selective antagonist by chemical library screening using an orthosteric radioligand binding assay were unsuccessful. Clone Assay:Article Title: Development of a beta-lactamase reporter gene assay for metabotropic glutamate receptor 1 by using coexpression of glutamate transporter. Article Snippet: Metabotropic glutamate receptors (mglurs) belong to a family of G-protein-coupled receptors and are thought to be involved in the modulation of neuronal excitability and neurotransmitter release.. Eight subtypes of the receptor (mGluR1-mGluR8) have been identified to date and are classified into 3 groups based on sequence homology, pharmacological profile, and signal transduction pathway. mGluR1 and mGluR5 are classified into group I mGluRs and are coupled to phospholipase C and subsequent intracellular calcium mobilization via Gq-protein.. Earlier efforts to identify an mGluR1-selective antagonist by chemical library screening using an orthosteric radioligand binding assay were unsuccessful. In Situ Hybridization:Article Title: Axon viability and mitochondrial function are dependent on local protein synthesis in sympathetic neurons. Article Snippet: 1) Axons contain numerous mRNAs and a local protein synthetic system that can be regulated independently of the cell body. (2) In this study, cultured primary sympathetic neurons were employed, to assess the effect of local protein synthesis blockade on axon viability and mitochondrial function. (3) Inhibition of local protein synthesis reduced newly synthesized axonal proteins by 65% and resulted in axon retraction after 6 h. Acute inhibition of local protein synthesis also resulted in a significant decrease in the membrane potential of axonal mitochondria.. Likewise, blockade of local protein transport into the mitochondria by transfection of the axons with Hsp90 C-terminal domain decreased the mitochondrial membrane potential by 65%.. Moreover, inhibition of the local protein synthetic system also reduced the ability of mitochondria to restore axonal levels of ATP after KCl-induced depolarization. (4) Taken together, these results indicate that the local protein synthetic system plays an important role in mitochondrial function and the maintenance of the axon. Reverse Transcription:Article Title: Axon viability and mitochondrial function are dependent on local protein synthesis in sympathetic neurons. Article Snippet: 1) Axons contain numerous mRNAs and a local protein synthetic system that can be regulated independently of the cell body. (2) In this study, cultured primary sympathetic neurons were employed, to assess the effect of local protein synthesis blockade on axon viability and mitochondrial function. (3) Inhibition of local protein synthesis reduced newly synthesized axonal proteins by 65% and resulted in axon retraction after 6 h. Acute inhibition of local protein synthesis also resulted in a significant decrease in the membrane potential of axonal mitochondria.. Likewise, blockade of local protein transport into the mitochondria by transfection of the axons with Hsp90 C-terminal domain decreased the mitochondrial membrane potential by 65%.. Moreover, inhibition of the local protein synthetic system also reduced the ability of mitochondria to restore axonal levels of ATP after KCl-induced depolarization. (4) Taken together, these results indicate that the local protein synthetic system plays an important role in mitochondrial function and the maintenance of the axon. Article Title: Identification and Cloning of Centaurin- α Article Snippet: Individual tryptic peptides were then subjected to automated Edman degradation on a Procise 492 protein sequencer (Perkin-Elmer, ABD division). .. The nine unique peptide sequences that were obtained were compared with sequences in the GenBank data base using the TFASTA and BLAST DNA analysis software package of the University of Wisconsin Genetics Computer Group. cDNA Cloning Rat brain template cDNA was prepared by reverse transcription of Article Title: Characterization of the adaptor protein ARH expression in the brain and ARH molecular interactions. Article Snippet: BioMax MR Films (Eastman Kodak, Rochester, NY, USA), were exposed for 72–96 h. In situ hybridization was conducted on cultured rat primary superior cervical ganglion (SCG) axons using a digoxigenin (DIG) labeled ARH riboprobe prepared as follows. .. ARH cDNA (GenBank accession’s number BC066808, nucleotides 54–580) was reverse transcribed from Article Title: Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats. Article Snippet: .. The entire open reading frame of Kv1.2 and Kv1.5 was amplified from Article Title: Identification and Cloning of Centaurin- α Article Snippet: .. Rat brain template cDNA was prepared by reverse transcription of Polymerase Chain Reaction:Article Title: Axon viability and mitochondrial function are dependent on local protein synthesis in sympathetic neurons. Article Snippet: 1) Axons contain numerous mRNAs and a local protein synthetic system that can be regulated independently of the cell body. (2) In this study, cultured primary sympathetic neurons were employed, to assess the effect of local protein synthesis blockade on axon viability and mitochondrial function. (3) Inhibition of local protein synthesis reduced newly synthesized axonal proteins by 65% and resulted in axon retraction after 6 h. Acute inhibition of local protein synthesis also resulted in a significant decrease in the membrane potential of axonal mitochondria.. Likewise, blockade of local protein transport into the mitochondria by transfection of the axons with Hsp90 C-terminal domain decreased the mitochondrial membrane potential by 65%.. Moreover, inhibition of the local protein synthetic system also reduced the ability of mitochondria to restore axonal levels of ATP after KCl-induced depolarization. (4) Taken together, these results indicate that the local protein synthetic system plays an important role in mitochondrial function and the maintenance of the axon. Article Title: Characterization of the adaptor protein ARH expression in the brain and ARH molecular interactions. Article Snippet: BioMax MR Films (Eastman Kodak, Rochester, NY, USA), were exposed for 72–96 h. In situ hybridization was conducted on cultured rat primary superior cervical ganglion (SCG) axons using a digoxigenin (DIG) labeled ARH riboprobe prepared as follows. .. ARH cDNA (GenBank accession’s number BC066808, nucleotides 54–580) was reverse transcribed from Article Title: Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats. Article Snippet: .. The entire open reading frame of Kv1.2 and Kv1.5 was amplified from Software:Article Title: Identification and Cloning of Centaurin- α Article Snippet: Individual tryptic peptides were then subjected to automated Edman degradation on a Procise 492 protein sequencer (Perkin-Elmer, ABD division). .. The nine unique peptide sequences that were obtained were compared with sequences in the GenBank data base using the TFASTA and BLAST DNA analysis software package of the University of Wisconsin Genetics Computer Group. cDNA Cloning Rat brain template cDNA was prepared by reverse transcription of Cloning:Article Title: Identification and Cloning of Centaurin- α Article Snippet: Individual tryptic peptides were then subjected to automated Edman degradation on a Procise 492 protein sequencer (Perkin-Elmer, ABD division). .. The nine unique peptide sequences that were obtained were compared with sequences in the GenBank data base using the TFASTA and BLAST DNA analysis software package of the University of Wisconsin Genetics Computer Group. cDNA Cloning Rat brain template cDNA was prepared by reverse transcription of Article Title: Voltage gated K+ channel expression in arteries of Wistar-Kyoto and spontaneously hypertensive rats. Article Snippet: .. The entire open reading frame of Kv1.2 and Kv1.5 was amplified from |